Difference between revisions of "Part:BBa K4156115:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
To be refined
 
  
 +
The AND gate is synthesized by nesting the cis-trans potential Bxb1 integrase and Tp901 integrase sites, enclosing two terminators in between. To ensure the smooth operation of the OR gate, we tested it using only the p7 promoter. The design of this part is based on 2015, Science, Drew Endy.
  
  
 
===Source===
 
===Source===
  
−
To be refined
+
Experimental constructed.
  
 
===References===
 
===References===
 +
 +
<i>
 +
Courbet A, Endy D, Renard E, Molina F, Bonnet J. Detection of pathological biomarkers in
 +
human clinical samples via amplifying genetic switches and logic gates. Sci Transl Med. May 27
 +
2015;7(289):289ra83. doi:10.1126/scitranslmed.aaa3601
 +
</i>

Latest revision as of 07:26, 9 October 2022


AND gate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 283
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 303
    Illegal BsaI.rc site found at 464


Design Notes

The AND gate is synthesized by nesting the cis-trans potential Bxb1 integrase and Tp901 integrase sites, enclosing two terminators in between. To ensure the smooth operation of the OR gate, we tested it using only the p7 promoter. The design of this part is based on 2015, Science, Drew Endy.


Source

Experimental constructed.

References

Courbet A, Endy D, Renard E, Molina F, Bonnet J. Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates. Sci Transl Med. May 27 2015;7(289):289ra83. doi:10.1126/scitranslmed.aaa3601