Difference between revisions of "Part:BBa K4156113:Design"
 
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===Design Notes===
 
===Design Notes===
  
−
plac-mRFP uses the classical red fluorescent protein (BBa_E1010) and the rrnB T1 and T7Te dual terminator (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_B0015"> BBa_B0015 </a></html>) to inter-assemble with the plac operon (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_R0010"> BBa_R0010 </a></html>). In addition, we introduced the RiboJ sequence after plac as well as RBS B0034 to optimize gene expression.
+
plac-mRFP uses the classical red fluorescent protein (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_E1010"> BBa_E1010 </a></html>) and the rrnB T1 and T7Te dual terminator (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_B0015"> BBa_B0015 </a></html>) to inter-assemble with the plac operon (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_R0010"> BBa_R0010 </a></html>). In addition, we introduced the RiboJ sequence after plac as well as RBS B0034 to optimize gene expression.
  
  

Latest revision as of 07:11, 9 October 2022


plac-mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 864
    Illegal AgeI site found at 976
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

plac-mRFP uses the classical red fluorescent protein ( BBa_E1010 ) and the rrnB T1 and T7Te dual terminator ( BBa_B0015 ) to inter-assemble with the plac operon ( BBa_R0010 ). In addition, we introduced the RiboJ sequence after plac as well as RBS B0034 to optimize gene expression.


Source

Experimental constructed.

References