Difference between revisions of "Part:BBa K4343096"
(→‎Reference)
Line 12: Line 12:
  
 
===Reference===
 
===Reference===
−
Li, M., Ou, X., Wei, D., Yang, X., Guo, D., Yian, X., & Xing, L. (2010). Cloning of delta8-fatty acid desaturase gene from Euglena gracilis and its expression in Saccharomyces cerevisiae. Sheng wu Gong Cheng xue bao= Chinese Journal of Biotechnology, 26(11), 1493-1499.
+
        Li, M., Ou, X., Wei, D., Yang, X., Guo, D., Yian, X., & Xing, L. (2010).Cloning of delta8-fatty acid desaturase gene from Euglena gracilis and its expression in Saccharomyces cerevisiae. Sheng wu Gong Cheng xue bao= Chinese Journal of Biotechnology, 26(11), 1493-1499.
  
  

Revision as of 07:06, 9 October 2022


EgDes8

EgDes8 encodes ∆-8 desaturase from Euglena gracilis which functions in the Δ-9 elongase/desaturase pathway and could convert C20:2 to C20:3.

Description

The EgDes8 gene encodes a ∆-8 desaturase of 422 amino acids from Euglena gracilis that catalyzes the conversion of eicosadienoic acid (EDA; C20: 2n-6) to dihomo-γ-linolenic acid (DGLA; C20: 3n-6).

Literature

The results showed that the high level of fatty acid desaturase could convert OMEGA-6-eicosanodienoic acid and OMEGA-3-EicosanoTRIenoic acid, and the substrate conversion rate was higher.

Reference

       Li, M., Ou, X., Wei, D., Yang, X., Guo, D., Yian, X., & Xing, L. (2010).Cloning of delta8-fatty acid desaturase gene from Euglena gracilis and its expression in Saccharomyces cerevisiae. Sheng wu Gong Cheng xue bao= Chinese Journal of Biotechnology, 26(11), 1493-1499.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 328
    Illegal XhoI site found at 649
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1286
    Illegal SapI.rc site found at 273