Difference between revisions of "Part:BBa K4156111"

 
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<partinfo>BBa_K4156111 short</partinfo>
 
<partinfo>BBa_K4156111 short</partinfo>
  
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pCaCd-mRFP is a biological brick that expresses mRFP under the control of the pCaCd promoter (BBa_K4156076). pCaCd-mRFP functions as a red fluorescent signal to characterize the performance of the pCaCd promoter.
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===Usage and Biology===
 
===Usage and Biology===
  
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In this biobrick, we used the pCaCd promoter to regulate downstream mRFP expression and eventually introduced the rrnB T1 and T7Te dual terminators. in addition, we added the RiboJ sequence and RBS B0034 for optimization. This design allowed the spatiotemporal intensity of gene expression under the regulation of the pCaCd promoter to be measured by the red fluorescent signal. We were thus able to verify the specific response of the pCaCd promoter to pH changes.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4156111 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4156111 SequenceAndFeatures</partinfo>

Revision as of 06:53, 9 October 2022


pCadC-mRFP

pCaCd-mRFP is a biological brick that expresses mRFP under the control of the pCaCd promoter (BBa_K4156076). pCaCd-mRFP functions as a red fluorescent signal to characterize the performance of the pCaCd promoter.


Usage and Biology

In this biobrick, we used the pCaCd promoter to regulate downstream mRFP expression and eventually introduced the rrnB T1 and T7Te dual terminators. in addition, we added the RiboJ sequence and RBS B0034 for optimization. This design allowed the spatiotemporal intensity of gene expression under the regulation of the pCaCd promoter to be measured by the red fluorescent signal. We were thus able to verify the specific response of the pCaCd promoter to pH changes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 731
    Illegal AgeI site found at 843
  • 1000
    COMPATIBLE WITH RFC[1000]