Difference between revisions of "Part:BBa K4156110:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
To be refined
+
 
 +
pPepT-mRFP uses the classical red fluorescent protein (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_E1010"> BBa_E1010 </a></html>) and the rrnB T1 and T7Te dual terminator (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_B0015"> BBa_B0015 </a></html>) to inter-assemble with the pPepT promoter (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156110"> BBa_K4156110 </a></html>) and introduces the RiboJ sequence as well as RBS B0034 after pPepT to optimize gene expression.
  
  
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===Source===
 
===Source===
  
−
To be refined
+
Experimental constructed.
  
 
===References===
 
===References===

Latest revision as of 06:50, 9 October 2022


pPepT-mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 723
    Illegal AgeI site found at 835
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pPepT-mRFP uses the classical red fluorescent protein ( BBa_E1010 ) and the rrnB T1 and T7Te dual terminator ( BBa_B0015 ) to inter-assemble with the pPepT promoter ( BBa_K4156110 ) and introduces the RiboJ sequence as well as RBS B0034 after pPepT to optimize gene expression.


Source

Experimental constructed.

References