Difference between revisions of "Part:BBa K4137008"

Revision as of 06:40, 9 October 2022

mleR expressing construct

This construct produces and purifies mleR, the malate-induced regulator that binds to p_mleS promoter for the production of ccdA antitoxin.

Fig.1 Malate-binding transcriptional activator + 6x His-Tag complete construct.

Construct Designs

We attached a 6x His-Tag downstream of the mleR sequence for purification purposes. A T7 and RBS (AAGGAG) are attached upstream to the side of the open reading frame. The terminator BBa_B1006 is attached downstream of the sequence.

Characterization

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 49
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 ===Construct Designs===
-We attached a 6x His-Tag downstream of the mleR sequence for purification purposes. A T7 and RBS are attached upstream to the side of the open reading frame. The terminator BBa_B1006 is attached downstream of the sequence.
+We attached a 6x His-Tag downstream of the mleR sequence for purification purposes. A T7 and RBS (AAGGAG) are attached upstream to the side of the open reading frame. The terminator BBa_B1006 is attached downstream of the sequence.
 ===Characterization===