Difference between revisions of "Part:BBa K4385025"

Revision as of 06:32, 9 October 2022

PbrR location detection

This component is made up of TurboID and PbrR. TurboID, a pair of biotin ligases, was designed to overcome the problem of slow existing biotin ligases.PbrR is a lead ion adsorption protein. The PbrR without absorbing lead ions was connected and formed a complex under TurbolD's traction. With the combination of the biotin and streptavdin-cy3, the fluorescent signal can be observed. So we can detect the quantity of the monomer pbrR protein which can combine with the turboid-pbrR fusion protein.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 1486
Illegal PstI site found at 1428
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 1486
Illegal PstI site found at 1428
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 1486
Illegal PstI site found at 1428
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 1486
Illegal PstI site found at 1428
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1219

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 <partinfo>BBa_K4385025 short</partinfo>
-&#12290;&#12290;
+This component is made up of TurboID and PbrR. TurboID, a pair of biotin ligases, was designed to overcome the problem of slow existing biotin ligases.PbrR is a lead ion adsorption protein. The PbrR without absorbing lead ions was connected and formed a complex under TurbolD's traction. With the combination of the biotin and streptavdin-cy3, the fluorescent signal can be observed. So we can detect the quantity of the monomer pbrR protein which can combine with the turboid-pbrR fusion protein.
 <!-- Add more about the biology of this part here