Difference between revisions of "Part:BBa K4343119"

Latest revision as of 05:22, 9 October 2022

LEU

LEU gene is also a commonly used screening marker in Y. lipolytica. Only strains with LEU gene can grow in the medium without Leu. Since the Y. lipolytica strains we used were Leu-deficient and the plasmid (BBa_K4343069) we constructed contained LEU gene. Only yeast cells that had been successfully introduced into the target segment with the LEU gene were able to grow on Leu-free medium. After screening, yeast cells containing both the target gene and the LEU gene were obtained. It is worth noting that in the present experiment the LEU screening marker cannot be lost and can only be used for a single transformation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 667
Illegal XhoI site found at 696
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1236
Illegal SapI site found at 1078

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 <partinfo>BBa_K4343119 short</partinfo>
-Only yeast cells that had been successfully introduced into the target segment with the LEU gene were able to grow on Leu-free medium. After screening, yeast cells containing both the target gene and the LEU gene were obtained. It is worth noting that in the present experiment the LEU screening marker cannot be lost and can only be used for a single transformation.
+LEU gene is also a commonly used screening marker in Y. lipolytica. Only strains with LEU gene can grow in the medium without Leu. Since the Y. lipolytica strains we used were Leu-deficient and the plasmid (BBa_K4343069) we constructed contained LEU gene. Only yeast cells that had been successfully introduced into the target segment with the LEU gene were able to grow on Leu-free medium. After screening, yeast cells containing both the target gene and the LEU gene were obtained. It is worth noting that in the present experiment the LEU screening marker cannot be lost and can only be used for a single transformation.
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