Difference between revisions of "Part:pSB3T5:Experience"
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[[Part:pSB3T5|pSB3T5]] is a functional low to medium copy plasmid but does not propagate at high copy.  [[Part:pSB3T5|pSB3T5]]-[[Part:BBa_I52001|I52001]] has been used to successfully assemble BioBrick parts.  However, [[Part:pSB3T5|pSB3T5]]-[[Part:BBa_I52001|I52001]] does not give high miniprep yields due to mutations in [[Part:BBa_I52001|BBa_I52001]] that render it nonfunctional as a high copy origin.  [[Part:pSB3T5|pSB3T5]]-[[Part:BBa_I52001|I52001]] works fine as a plasmid but is not as convenient for plasmid DNA purification.
 
[[Part:pSB3T5|pSB3T5]] is a functional low to medium copy plasmid but does not propagate at high copy.  [[Part:pSB3T5|pSB3T5]]-[[Part:BBa_I52001|I52001]] has been used to successfully assemble BioBrick parts.  However, [[Part:pSB3T5|pSB3T5]]-[[Part:BBa_I52001|I52001]] does not give high miniprep yields due to mutations in [[Part:BBa_I52001|BBa_I52001]] that render it nonfunctional as a high copy origin.  [[Part:pSB3T5|pSB3T5]]-[[Part:BBa_I52001|I52001]] works fine as a plasmid but is not as convenient for plasmid DNA purification.
 
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Revision as of 02:44, 23 September 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of pSB3T5

User Reviews

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••••

Reshma Shetty

pSB3T5 is a functional low to medium copy plasmid but does not propagate at high copy. pSB3T5-I52001 has been used to successfully assemble BioBrick parts. However, pSB3T5-I52001 does not give high miniprep yields due to mutations in BBa_I52001 that render it nonfunctional as a high copy origin. pSB3T5-I52001 works fine as a plasmid but is not as convenient for plasmid DNA purification.

••••

Aberdeen_Scotland 2009

Plasmid rescued contained BBa_J04450. Transformation, miniprep, double and single digests all worked properly. It was noticeable that colonies were a light pink colour. We used this part for further cloning. This plasmid gave us a very high efficiency of cloning.

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