Difference between revisions of "Part:BBa K4137000"

(→‎Sequence and Features)
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[[Image:ccda-his.png|800px|thumb|center|Fig.1 ccdA with C-Terminal 6x His-Tag.]]
 
[[Image:ccda-his.png|800px|thumb|center|Fig.1 ccdA with C-Terminal 6x His-Tag.]]
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===Characterization===
  
 
===Sequence and Features===
 
===Sequence and Features===

Revision as of 04:07, 9 October 2022


CcdA-6xHis Antitoxin

BBa_K4137000 codes for the CcdA protein, a component of the CcdA-CcdB toxin-antitoxin system. CcdA binds with CcdB which inhibits CcdB from targeting DNA gyrase thus preventing DNA double-strand breaks. A 6xHis tag was added for protein purification and identification.


Fig.1 ccdA with C-Terminal 6x His-Tag.

Characterization

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]