Difference between revisions of "Part:BBa K4137001"
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[[File:ccdb-myc.png|800px|thumb|center|Fig.1 ccdB with C-Terminal Myc-Tag.]]
 
[[File:ccdb-myc.png|800px|thumb|center|Fig.1 ccdB with C-Terminal Myc-Tag.]]
  
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===Sequence and Features===
 
===Sequence and Features===
  

Revision as of 04:06, 9 October 2022


CcdB-Myc Toxin

BBa_K4137001 codes for the CcdB protein, a toxin to many bacteria including E. coli and B. subtilis. Through the formation of a CcdA-CcdB complex by binding with CcdA, CcdB loses its ability to interact with DNA gyrase thus losing its toxicity. A Myc tag is added for protein purification, identification, and distinction from CcdA.

Fig.1 ccdB with C-Terminal Myc-Tag.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 216