Difference between revisions of "Part:BBa K4156123:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
To be refined
+
The OR gate is synthesized by nesting the cis Bxb1 integrase and cis Tp901 integrase sites, enclosing a terminator B0015 in between. to ensure the smooth operation of the OR gate, we tested it using only the p7 promoter. The design of this part is based on 2015, Science, Drew Endy.
−
 
+
  
  
 
===Source===
 
===Source===
  
−
To be refined
+
Experimental Constructed
  
 
===References===
 
===References===
 +
<i>
 +
Courbet A, Endy D, Renard E, Molina F, Bonnet J. Detection of pathological biomarkers in
 +
human clinical samples via amplifying genetic switches and logic gates. Sci Transl Med. May 27
 +
2015;7(289):289ra83. doi:10.1126/scitranslmed.aaa3601
 +
</i>

Latest revision as of 03:08, 9 October 2022


OR gate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 104
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 124
    Illegal BsaI site found at 306


Design Notes

The OR gate is synthesized by nesting the cis Bxb1 integrase and cis Tp901 integrase sites, enclosing a terminator B0015 in between. to ensure the smooth operation of the OR gate, we tested it using only the p7 promoter. The design of this part is based on 2015, Science, Drew Endy.


Source

Experimental Constructed

References

Courbet A, Endy D, Renard E, Molina F, Bonnet J. Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates. Sci Transl Med. May 27 2015;7(289):289ra83. doi:10.1126/scitranslmed.aaa3601