Difference between revisions of "Part:BBa K4137000:Design"
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<partinfo>BBa_K4137000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4137000 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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An alternative version, BBa_K4137010, is the CcdA sequence with the Myc tag added to the N-terminal of the protein. | An alternative version, BBa_K4137010, is the CcdA sequence with the Myc tag added to the N-terminal of the protein. | ||
| + | [Image:ccda-his.png|750px|thumb|center|Figure 1. ccdA with a C terminal 6xHis Tag.]] | ||
===Source=== | ===Source=== | ||
Revision as of 03:00, 9 October 2022
CcdA-6xHis Antitoxin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The 6xHis purification tag was added to the C-terminal of the protein. The sequence has been codon optimized for E. coli. An alternative version, BBa_K4137010, is the CcdA sequence with the Myc tag added to the N-terminal of the protein.
[Image:ccda-his.png|750px|thumb|center|Figure 1. ccdA with a C terminal 6xHis Tag.]]
Source
https://www.ncbi.nlm.nih.gov/nuccore/U51588.1