Difference between revisions of "Part:BBa K4156123"

Revision as of 03:00, 9 October 2022

OR gate

OR gate is a type of Boolean logic gate that can be used to activate downstream gene expression in response to an upstream counterpart promoter in the presence of any or all markers.

Usage and Biology

The OR gate is constructed with a p7 promoter and a terminator ( BBa_B0015 ). We added all cis Bxb1 integrase and Tp901 integrase sites on both sides of the terminator to form the so-called OR gate. Finally, we introduced RiboJ and BCD2 sequences to ensure smooth transcription with the OR gate on.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 104
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 124
Illegal BsaI site found at 306

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 <partinfo>BBa_K4156123 short</partinfo>
-To be refined
+OR gate is a type of Boolean logic gate that can be used to activate downstream gene expression in response to an upstream counterpart promoter in the presence of any or all markers.
+<!-- Add more about the biology of this part here -->
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The OR gate is constructed with a p7 promoter and a terminator (<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_B0015"> BBa_B0015 </a></html>). We added all cis Bxb1 integrase and Tp901 integrase sites on both sides of the terminator to form the so-called OR gate. Finally, we introduced RiboJ and BCD2 sequences to ensure smooth transcription with the OR gate on.
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K4156123 SequenceAndFeatures</partinfo>