Difference between revisions of "Part:BBa K143057"
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#1 https://parts.igem.org/Assembly:RBS-CDS_issues | #1 https://parts.igem.org/Assembly:RBS-CDS_issues | ||
</biblio> | </biblio> | ||
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+ | ==Contribution:iGEM22_WHU-China== | ||
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+ | <div class="col-lg" style="margin:auto;text-align:center;"> | ||
+ | <img style="margin:20px auto 5px auto;" src="https://static.igem.wiki/teams/4144/wiki/same-part/bba-k4144004/picture1.png" width="60%"> | ||
+ | <p style="color:Gray; padding:0px 30px 10px;">Figure. 1 Expression of Pet8p in E. coli and transportation efficiency measurement in liposome</p> | ||
+ | </div> | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display |
Revision as of 02:37, 9 October 2022
Promoter xyl and RBS spoVG for B subtilis
Inducible promoter xyl(BBa_K143014) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis.
Pxyl-spoVG can be used to take an input of xylose and give a Ribosomes per second (RiPS) output generator.
Xylose does not directly induce the expression of the promoter xyl, but requires the transcriptional regulator XylR, (BBa_K143036). This means that XylR must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter. XylR is naturally expressed by B. subtilis but should be upregulated to increase efficiency.
To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
<biblio>
</biblio>
Contribution:iGEM22_WHU-China
Figure. 1 Expression of Pet8p in E. coli and transportation efficiency measurement in liposome