Difference between revisions of "Part:BBa K4147004"

Revision as of 02:35, 9 October 2022

DrsB1 Construct with LacI Promoter

Antimicrobial protein from the arboreal tree frog Phyllomedusa bicolor, dermaseptin-b1 gene (AKA MsrA2) with added parts as a single sequence to promote expression, being optimized for E. coli. Other than the original sequence for MsrA2 (BBa_K2577001), we added a LacI promoter (BBa_R0010), an RBS (BBa_B0032) and a double terminator (BBa_B0015).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 01:53, 1 October 2022 (view source) Registry (Talk \| contribs)		Revision as of 02:35, 9 October 2022 (view source) Ana Belem (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K4147004 short</partinfo>		<partinfo>BBa_K4147004 short</partinfo>

−	Antimicrobial protein from the arboreal tree frog Phyllomedusa bicolor, dermaseptin-b1 gene (AKA MsrA2) with ~~addded~~ parts as a single sequence to promote expression, being optimized for E. coli. Other than the original sequence for MsrA2 (BBa_K2577001), we added a LacI promoter (BBa_R0010), a RBS (BBa_B0032) and a double terminator (BBa_B0015).	+	Antimicrobial protein from the arboreal tree frog <i>Phyllomedusa bicolor</i>, dermaseptin-b1 gene (AKA MsrA2) with added parts as a single sequence to promote expression, being optimized for E. coli. Other than the original sequence for MsrA2 (BBa_K2577001), we added a LacI promoter (BBa_R0010), an RBS (BBa_B0032) and a double terminator (BBa_B0015).