Difference between revisions of "Part:BBa K4147003:Design"

 
 
Line 15: Line 15:
 
GenBank: EU271754.1
 
GenBank: EU271754.1
 
https://www.ncbi.nlm.nih.gov/nuccore/EU271754.1
 
https://www.ncbi.nlm.nih.gov/nuccore/EU271754.1
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===References===
 

Latest revision as of 02:32, 9 October 2022


PcOSM Construct with LacI regulated promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 806
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 498
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When sending the part to synthetize, be sure to add some extra bases at the ends of the prefix and suffix, otherwise, the restriction enzymes might not be able to digest, making it difficult to later ligate in a vector.


Source

GenBank: EU271754.1 https://www.ncbi.nlm.nih.gov/nuccore/EU271754.1