Difference between revisions of "Part:BBa K4147002:Design"
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===DESIGN NOTES=== | ===DESIGN NOTES=== | ||
It is widely use in eukaryotic cells. In addition, as ist is an antimicrobial peptide is desired to express it under medium strenght promoter, in order to mantain the concentration of a toxic target protein just below a host strain's tolerance. The PelB sequence allows the peptide to be sent to a periplasmic space for easier purification and an histidine tag for IMAC purification. | It is widely use in eukaryotic cells. In addition, as ist is an antimicrobial peptide is desired to express it under medium strenght promoter, in order to mantain the concentration of a toxic target protein just below a host strain's tolerance. The PelB sequence allows the peptide to be sent to a periplasmic space for easier purification and an histidine tag for IMAC purification. | ||
Latest revision as of 02:32, 9 October 2022
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 392
Illegal AgeI site found at 671 - 1000COMPATIBLE WITH RFC[1000]
DESIGN NOTES
It is widely use in eukaryotic cells. In addition, as ist is an antimicrobial peptide is desired to express it under medium strenght promoter, in order to mantain the concentration of a toxic target protein just below a host strain's tolerance. The PelB sequence allows the peptide to be sent to a periplasmic space for easier purification and an histidine tag for IMAC purification.
SOURCE
This sequence was obtained from the GenBank (MW048779.1) as a synthetic construct fot th production of a recombinant Dermaseptin peptide in Nicotiana tabacum hairy roots with enhanced antimicrobial activity.