Difference between revisions of "Part:BBa K4242005"
Jiangzhelin (Talk | contribs) (→Usage and Biology) |
Jiangzhelin (Talk | contribs) (→Usage and Biology) |
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We constructed the composite part (BBa_K4242017) to control the intracellular c-di-GMP level in our engineering strain. In that part, the expression of <i>yhjH</i> is controlled by Ptac. To eliminate the effects from other place of the biobrick, we constructed this part and inserted it into the wide-type strain as a control. | We constructed the composite part (BBa_K4242017) to control the intracellular c-di-GMP level in our engineering strain. In that part, the expression of <i>yhjH</i> is controlled by Ptac. To eliminate the effects from other place of the biobrick, we constructed this part and inserted it into the wide-type strain as a control. | ||
<h3><center>pHYD-1</center></h3> | <h3><center>pHYD-1</center></h3> | ||
| − | [[Image:PHYD-1-1.png|centre| | + | [[Image:PHYD-1-1.png|centre|700px]] |
<center><b>Figure 1:</b>To test pHYD-1.</center> | <center><b>Figure 1:</b>To test pHYD-1.</center> | ||
Revision as of 02:30, 9 October 2022
pSB3C5-A1
This part has Ptac coding region and a double terminator TT. We use this part as a blank control to test our biosensor’s performance in different bacteria.
Usage and Biology
We constructed the composite part (BBa_K4242017) to control the intracellular c-di-GMP level in our engineering strain. In that part, the expression of yhjH is controlled by Ptac. To eliminate the effects from other place of the biobrick, we constructed this part and inserted it into the wide-type strain as a control.
pHYD-1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]