Difference between revisions of "Part:BBa K4432020"

 
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This part is an ''Escherichia coli'' codon optimized version of the ß-carotene-ketolase (CrtW148) from ''Nostoc punctiforme'' PCC 73102 (NCBI Reference Sequence: WP_012412154.1).
  
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===Usage and Biology===
 
===Usage and Biology===
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The ß-carotene-ketolase (EC 1.14.99.63) catalyses the conversion of ß-carotene into echinenone and of echinenone into canthaxanthin (Figure 1).
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[[File:T--Evry_Paris-Saclay--reaction_carotene-canthaxanthine.png|900px|thumb|left| Figure 1. Conversion of ß-carotene into canthaxanthin by EC: 1.14.99.63.]]
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The ''Nostoc punctiforme'' CrtW [1] is a 30.743 kDa protein of 262 amino acids.
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When placed under the control of the hybrid T5 promoter regulated by LacI ([[Part:BBa_K4432000|BBa_K4432000]]) or the classical pLac promoter ([[Part:BBa_R0010|BBa_R0010]]), this part leads to the synthesis of canthaxanthin in an ''E. coli'' producing ß-carotene.
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For further details, see the Main Pages on the Registry of the CrtW expression plasmids ([[Part:BBa_K4432120|BBa_K4432120]], [[Part:BBa_K4432220|BBa_K4432220]] and [[Part:BBa_K4432320|BBa_K4432320]]).
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===References===
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[1] Steiger S, Sandmann G. Cloning of two carotenoid ketolase genes from ''Nostoc punctiforme'' for the heterologous production of canthaxanthin and astaxanthin. Biotechnology Letters (2004) 26: 813–817.
  
 
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Revision as of 21:25, 8 October 2022


CrtW: β−carotene ketolase

This part is an Escherichia coli codon optimized version of the ß-carotene-ketolase (CrtW148) from Nostoc punctiforme PCC 73102 (NCBI Reference Sequence: WP_012412154.1).

Usage and Biology

The ß-carotene-ketolase (EC 1.14.99.63) catalyses the conversion of ß-carotene into echinenone and of echinenone into canthaxanthin (Figure 1).

Figure 1. Conversion of ß-carotene into canthaxanthin by EC: 1.14.99.63.

The Nostoc punctiforme CrtW [1] is a 30.743 kDa protein of 262 amino acids.

When placed under the control of the hybrid T5 promoter regulated by LacI (BBa_K4432000) or the classical pLac promoter (BBa_R0010), this part leads to the synthesis of canthaxanthin in an E. coli producing ß-carotene.

For further details, see the Main Pages on the Registry of the CrtW expression plasmids (BBa_K4432120, BBa_K4432220 and BBa_K4432320).

References

[1] Steiger S, Sandmann G. Cloning of two carotenoid ketolase genes from Nostoc punctiforme for the heterologous production of canthaxanthin and astaxanthin. Biotechnology Letters (2004) 26: 813–817.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]