Difference between revisions of "Part:BBa K4321005"
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This plasmid can replicate in both E.coli and B.subtilis and can be used when B.subtilis is to be used as the destination bacteria.
 
This plasmid can replicate in both E.coli and B.subtilis and can be used when B.subtilis is to be used as the destination bacteria.
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4321005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4321005 SequenceAndFeatures</partinfo>
  

Revision as of 21:17, 8 October 2022


E.coli to Bacillus subtilis shuttle plasmid (pCG004)

pCG004 is an E.coli - Bacillus subtilis shuttle plasmid that was created based off of the pHT01 plasmid. This plasmid contains a dropout GFP that can be replaced with the insert of choice.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9048
    Illegal suffix found in sequence at 1
    Illegal EcoRI site found at 3615
    Illegal EcoRI site found at 6835
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3615
    Illegal EcoRI site found at 6835
    Illegal EcoRI site found at 9048
    Illegal NheI site found at 3451
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 9054
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3615
    Illegal EcoRI site found at 6835
    Illegal EcoRI site found at 9048
    Illegal BglII site found at 361
    Illegal BglII site found at 6306
    Illegal XhoI site found at 365
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9048
    Illegal suffix found in sequence at 2
    Illegal EcoRI site found at 3615
    Illegal EcoRI site found at 6835
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9048
    Illegal EcoRI site found at 3615
    Illegal EcoRI site found at 6835
    Illegal XbaI site found at 9063
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6234
    Illegal BsaI.rc site found at 5170
    Illegal SapI.rc site found at 2338
    Illegal SapI.rc site found at 7459


References

PCG004 (plasmid #87377). Addgene. (n.d.). Retrieved October 8, 2022, from https://www.addgene.org/87377/

Tran, D. T., Phan, T. T., Doan, T. T., Tran, T. L., Schumann, W., & Nguyen, H. D. (2020). Integrative expression vectors with Pgrac promoters for inducer-free overproduction of recombinant proteins in bacillus subtilis. Biotechnology Reports, 28. https://doi.org/10.1016/j.btre.2020.e00540