Difference between revisions of "Part:BBa K4268003:Experience"
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| − | [[File: T-suny-oneonta-t7-capsid protein with biotin tag colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of | + | [[File: T-suny-oneonta-t7-capsid protein with biotin tag colony PCR figure.png|500px|thumb|center|Figure 1: Colony PCR of five colonies (clones) suspected of containing the Capsid Protein with Biotin Tag insert. The insert length is 780bp and the predicted size of the PCR product when using VF/VR primers is 1085 bp.]] |
The gel indicates that colonies 1, 2, and 3 are likely to contain the correct insert and thus, the Capsid Protein with Biotin Tag was successfully cloned into a Level 0 Golden Gate Assembly basic part. | The gel indicates that colonies 1, 2, and 3 are likely to contain the correct insert and thus, the Capsid Protein with Biotin Tag was successfully cloned into a Level 0 Golden Gate Assembly basic part. | ||
Latest revision as of 18:22, 8 October 2022
The part was received, resuspended, and cloned into the level 0 Golden Gate vector, PSB1C00. The ligation was then transformed into DH5α cells. After overnight growth, white colonies (indicative of a replacement of the RFP reporter cassette) were subjected to screening for the correct insert using colony PCR with the VF/VR primer pair.
The gel indicates that colonies 1, 2, and 3 are likely to contain the correct insert and thus, the Capsid Protein with Biotin Tag was successfully cloned into a Level 0 Golden Gate Assembly basic part.
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UNIQc6dcc53c6d053f7c-partinfo-00000000-QINU UNIQc6dcc53c6d053f7c-partinfo-00000001-QINU