Difference between revisions of "Part:BBa K4345023"

 
 
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Design of a thermoregulated translation of antitoxin ccdA. Fluorescent protein sfGFP was linked to the antitoxin with a rigid linker for visualisation.  
 
Design of a thermoregulated translation of antitoxin ccdA. Fluorescent protein sfGFP was linked to the antitoxin with a rigid linker for visualisation.  
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4345023 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4345023 SequenceAndFeatures</partinfo>
  
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===Usage and Biology===
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The figure below shows the secondary structure of the RNA thermometer.
  
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[[Image:RpoH Morita et al 1999.jpeg|300px]]
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===Functional Parameters===
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<partinfo>BBa_K4345023 parameters</partinfo>
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Image obtained from Morita et al., 1999.
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===References===
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Morita, M. T., Tanaka, Y., Kodama, T. S., Kyogoku, Y., Yanagi, H., & Yura, T. (1999, March 15). Translational induction of heat shock transcription factor sigma 32: evidence for a built-in RNA thermosensor. Genes &Amp; Development, 13(6), 655–665. https://doi.org/10.1101/gad.13.6.655

Latest revision as of 17:58, 8 October 2022


5' UTR of rpoH with ccdA fused to sfGFP

Design of a thermoregulated translation of antitoxin ccdA. Fluorescent protein sfGFP was linked to the antitoxin with a rigid linker for visualisation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1347
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 767
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

The figure below shows the secondary structure of the RNA thermometer.

RpoH Morita et al 1999.jpeg

Image obtained from Morita et al., 1999.


References

Morita, M. T., Tanaka, Y., Kodama, T. S., Kyogoku, Y., Yanagi, H., & Yura, T. (1999, March 15). Translational induction of heat shock transcription factor sigma 32: evidence for a built-in RNA thermosensor. Genes &Amp; Development, 13(6), 655–665. https://doi.org/10.1101/gad.13.6.655