Difference between revisions of "Part:BBa K4174001"
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===Biological Relevance=== | ===Biological Relevance=== | ||
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| + | The ability to assay whether a chassis is actively transcribing its circuit to make proteins is crucial for testing the efficacy of a fieldable construct. Bacteria have two main life stages - exponential growth, during which they reproduce and express their circuits with ease, and stationary phase, during which they cease most non-essential metabolic activity. Since stationary phase is induced by inopportune environments, such as metabolic shortage, most bacteria in nature exist in stationary phase (Jaishankar 2000). This is a major roadblock for fieldable synthetic biology, as constructs that work perfectly in the lab may stop expressing their circuit when introduced into their deployment sites. In order to assay how a circuit will behave in nature, constructs should be tested in the lab while in stationary phase. | ||
===Design Notes=== | ===Design Notes=== | ||
Revision as of 16:53, 8 October 2022
osmY-mRFP1
This composite part is composed of osmY promoter, the B0030 RBS, mRFP1, and the B0015 terminator.
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 823
Illegal AgeI site found at 935 - 1000COMPATIBLE WITH RFC[1000]
Biological Relevance
The ability to assay whether a chassis is actively transcribing its circuit to make proteins is crucial for testing the efficacy of a fieldable construct. Bacteria have two main life stages - exponential growth, during which they reproduce and express their circuits with ease, and stationary phase, during which they cease most non-essential metabolic activity. Since stationary phase is induced by inopportune environments, such as metabolic shortage, most bacteria in nature exist in stationary phase (Jaishankar 2000). This is a major roadblock for fieldable synthetic biology, as constructs that work perfectly in the lab may stop expressing their circuit when introduced into their deployment sites. In order to assay how a circuit will behave in nature, constructs should be tested in the lab while in stationary phase.