Difference between revisions of "Part:BBa K4239007"

 
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<h2>Sequence and Features</h2>
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<partinfo>BBa_K4239007 SequenceAndFeatures</partinfo>
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<h2>Description</h2>
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<p><i>fiatluxA</i> is made to be used with <i>fiatluxB</i>. It codes for a subpart of the luciferase protein. With the subpart coding from <i>fiatluxB</i>, they form the luciferase protein.</p>
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<p>Luciferase has as substrats FMNH2, O2 and Fatty aldehydes, and produces H20, Fatty Acids and FMN and emits luminescence.</p>
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<p>The systeme <i>fiatluxA/fiatluxB</i> is made to be used with <i>fiatluxC, fiatluxD and fiatluxE</i>, gathered in the <i>fiatluxCDABE</i> operon.</p>
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<p><i>Fiatlux</i> genes come from <i>ilux</i> genes (C, D, A, B, E). They were modified to remove every Igem restriction site (EcoR1, Xba1, Spe1 and Pst1) included in genes. They were also adapted to include the biobrick format.</p>
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<p>The <i>ilux operon</i> was born from a mutated natural luminescence operon present in the bacteria P.luminescens: the <i>lux</i> operon. These mutations were error-prone PCR induced according to Gregor et al.’s study in 2018 (Gregor et al. 2018). The aim was to create a system of genes that produced more light.</p>
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<h2>Construction</h2>
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resultats de l'overlap PCR , j'ai pas trop compris ce qui a était fait...!!!!!!!!!!!!!!!!!
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caractérisation dans E.coli DH5alpha...!!!!!!!!!!!!!!
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<h2>References</h2>
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<p><i> Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.</i> </p>
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Revision as of 16:40, 8 October 2022


Enhanced units fiatluxABE

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2853
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 504
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1023

Description

fiatluxA is made to be used with fiatluxB. It codes for a subpart of the luciferase protein. With the subpart coding from fiatluxB, they form the luciferase protein.

Luciferase has as substrats FMNH2, O2 and Fatty aldehydes, and produces H20, Fatty Acids and FMN and emits luminescence.

The systeme fiatluxA/fiatluxB is made to be used with fiatluxC, fiatluxD and fiatluxE, gathered in the fiatluxCDABE operon.

Fiatlux genes come from ilux genes (C, D, A, B, E). They were modified to remove every Igem restriction site (EcoR1, Xba1, Spe1 and Pst1) included in genes. They were also adapted to include the biobrick format.

The ilux operon was born from a mutated natural luminescence operon present in the bacteria P.luminescens: the lux operon. These mutations were error-prone PCR induced according to Gregor et al.’s study in 2018 (Gregor et al. 2018). The aim was to create a system of genes that produced more light.

Construction

resultats de l'overlap PCR , j'ai pas trop compris ce qui a était fait...!!!!!!!!!!!!!!!!! caractérisation dans E.coli DH5alpha...!!!!!!!!!!!!!!

References

Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.