Difference between revisions of "Part:BBa K4239001"
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<h2>Description</h2>  
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<h2>Construction</h2>  
  
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<p><i>fiatluxC</i> was synthesized with <i>fiatluxD</i> and the promoter J23117<a href="https://parts.igem.org/Part:BBa_J23117" class="pr-0" target="_blank">(BBa_J23117)</a>
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<p><i>fiatluxC</i> was directly synthesized with <i>fiatluxD</i> and the promoter J23117 <a href="https://parts.igem.org/Part:BBa_J23117" class="pr-0" target="_blank">(BBa_J23117)</a>. The description of the synthesis is on the page part BBa_K4239006 <a href="https://parts.igem.org/Part:BBa_K4239006" class="pr-0" target="_blank">(BBa_K4239006)</a>.
  
 
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</p>

Revision as of 15:35, 8 October 2022


Enhanced luciferase subunits fiatluxC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1311

Description

fiatluxC is made to be used with fiatluxD and fiatluxE. It codes for a subpart fatty acid reductase. With the subparts coding from fiatluxD and fiatluxE, they form a complex that recycles fatty acids to fatty aldehydes. Fatty aldehydes will be used as a substrat for the luciferase protein.

The systeme fiatluxC/fiatluxD/fiatluxE is made to be used with fiatluxA and fiatluxB, gathered in the fiatluxCDABE operon.

Fiatlux genes come from ilux genes (C, D, A, B, E). They were modified to remove every Igem restriction site (EcoR1, Xba1, Spe1 and Pst1) included in genes. They were also adapted to include the biobrick format.

The ilux operon was born from a mutated natural luminescence operon present in the bacteria P.luminescens: the lux operon. These mutations were error-prone PCR induced according to Gregor et al.’s study in 2018 (Gregor et al. 2018). The aim was to create a system of genes that produced more light.

Construction

fiatluxC was directly synthesized with fiatluxD and the promoter J23117 (BBa_J23117). The description of the synthesis is on the page part BBa_K4239006 (BBa_K4239006).

METTRE LES PRIMERS UTILISés