Difference between revisions of "Part:BBa K4122020"
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<p>When replacing RFP and GFP with MHETase and PETase, we did not observe immunofluorescence with secondary antibodies that should theoretically bind specifically to the V5 tag.</p> | <p>When replacing RFP and GFP with MHETase and PETase, we did not observe immunofluorescence with secondary antibodies that should theoretically bind specifically to the V5 tag.</p> | ||
<p>Then we started to analyze.</p > | <p>Then we started to analyze.</p > | ||
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| + | <img src="https://static.igem.wiki/teams/4122/wiki/parts/10-the-construction-of-plasmid-ts-pgapdh-teno1a-the-surface-display-system-for-displaying-both-petase-and-mhetase.png" style = "width:70%;"> | ||
| + | <br/><b>Fig.1</b> The construction of plasmid Ts-PGAPDH--TENO1A, the surface display system for displaying both PETase and MHETase. | ||
| + | <br/> | ||
Latest revision as of 15:05, 8 October 2022
SP-MHETase-cMyc-Snooptag+SP-PETase-His-Spytag+SP-CBM-SC-SC-SNC-SC-V5-7813
This parts is composed of 3 PARTS:
PFBA1-SP-PETase-His-Spytag-T-ADH2
PFBA1-SP-MHETase-cMyc-Snooptag-T-PGK1
SP-CBM-SC-SC-SNC-SC-V5-7813
When replacing RFP and GFP with MHETase and PETase, we did not observe immunofluorescence with secondary antibodies that should theoretically bind specifically to the V5 tag.
Then we started to analyze.
Fig.1 The construction of plasmid Ts-PGAPDH--TENO1A, the surface display system for displaying both PETase and MHETase.
Sequence and Features