Difference between revisions of "Part:BBa K4339000:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
Since Cyc A originates in a K-12 strain of E. coli, modifications and codon optimisation had to be performed to make the gene more compatible with our vector strain, which is a BL21 derivative strain.
+
The sequence was modified to remove forbidden restriction sites to allow for type IIS cloning.
  
 
===Source===
 
===Source===

Revision as of 14:58, 8 October 2022


Non-polar amino acid transporter (CycA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 340
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was modified to remove forbidden restriction sites to allow for type IIS cloning.

Source

The Cyc A sequence originates in a K-12 strain of e. Coli.

References