Difference between revisions of "Part:BBa K228866:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | part | + | when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation. |
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===Source=== | ===Source=== | ||
Revision as of 10:14, 22 September 2009
AND Gate pBad-SupD pSal-PhiR73 delta(amber mutation)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1274
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2007
Illegal BamHI site found at 1214 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1839
Illegal AgeI site found at 1320 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1323
Design Notes
when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.
Source
when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.