Difference between revisions of "Part:BBa K4345019"

 
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<partinfo>BBa_K4345019 short</partinfo>
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In nature, rpoH is implemented in the mRNA structure of a Escherichia Coli heatshock gene which is important for stress tolerance.
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The thermometer is a structural element that is located within the 5’ UTR of protein-coding mRNA. It operates as a reversible molecular zipper that controls the availability of the RBS in its structure. When the temperature increases, the thermometer unzips and the RBS can be detected by the ribosome thus allowing translation of downstream mRNA. When the temperature is below a certain range, the RBS gets trapped.
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===Sequence and Features===
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<partinfo>BBa_K4345019 SequenceAndFeatures</partinfo>
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===Usage and Biology===
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The figure below shows the secondary structure of the RNA thermometer.
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[[Image:RpoH Morita et al 1999.jpeg|300px]]
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Image obtained from Morita et al., 1999.
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===References===
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Morita, M. T., Tanaka, Y., Kodama, T. S., Kyogoku, Y., Yanagi, H., & Yura, T. (1999, March 15). Translational induction of heat shock transcription factor sigma 32: evidence for a built-in RNA thermosensor. Genes &Amp; Development, 13(6), 655–665. https://doi.org/10.1101/gad.13.6.655

Revision as of 14:34, 8 October 2022


RNA Thermometer rpoH

In nature, rpoH is implemented in the mRNA structure of a Escherichia Coli heatshock gene which is important for stress tolerance. The thermometer is a structural element that is located within the 5’ UTR of protein-coding mRNA. It operates as a reversible molecular zipper that controls the availability of the RBS in its structure. When the temperature increases, the thermometer unzips and the RBS can be detected by the ribosome thus allowing translation of downstream mRNA. When the temperature is below a certain range, the RBS gets trapped.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

The figure below shows the secondary structure of the RNA thermometer.

RpoH Morita et al 1999.jpeg

Image obtained from Morita et al., 1999.


References

Morita, M. T., Tanaka, Y., Kodama, T. S., Kyogoku, Y., Yanagi, H., & Yura, T. (1999, March 15). Translational induction of heat shock transcription factor sigma 32: evidence for a built-in RNA thermosensor. Genes &Amp; Development, 13(6), 655–665. https://doi.org/10.1101/gad.13.6.655