Difference between revisions of "Part:BBa K4361007"

 
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Based off of BBa_K4361001. Since that part's second inverted repeat pair consists of only 5 nucleotides on each end of the region, the region marked "Inverted repeat pair 1" was adjusted to only have the outer 5 nucleotides be the inverted repeat while the middlemost nucleotides have been replaced by pair 2's non-repeating sequence.
 
Based off of BBa_K4361001. Since that part's second inverted repeat pair consists of only 5 nucleotides on each end of the region, the region marked "Inverted repeat pair 1" was adjusted to only have the outer 5 nucleotides be the inverted repeat while the middlemost nucleotides have been replaced by pair 2's non-repeating sequence.
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4361007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4361007 SequenceAndFeatures</partinfo>
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<h3>Usage and biology</h3>
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<html>
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<h3>Results</h3>
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~~~
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<figure>
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<a href="https://static.igem.wiki/teams/4361/wiki/parts/oligos-07.png"><img src="https://static.igem.wiki/teams/4361/wiki/parts/oligos-07.png" style="width:300px;margin-left:275px"></a>
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<figcaption> <b>Figure 2.</b> Results of the second Tapestation experiment, in which the fraction of DNA bound to BlcR was determined for different types of oligos. The first bar and bottom dashed line represent the results with </html>[[Part:BBa_K4361000]]<html> (scrambled oligo, negative control), the second bar and top dashed line correspond to those with </html>[[Part:BBa_K4361001]]<html> (wildtype oligo, positive control). The third bar depicts the measured fraction of bound DNA for this part.</figcaption>
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</figure>
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<figure>
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<a href="https://static.igem.wiki/teams/4361/wiki/parts/add-ssa-07.png"><img src="https://static.igem.wiki/teams/4361/wiki/parts/add-ssa-07.png" style="width:300px;margin-left:275px"></a>
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<figcaption> <b>Figure 3.</b> Results of the third Tapestation experiment, in which the fraction of DNA bound to BlcR was determined for different types of oligos in the
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<span style="color:#4CCAF1;">presence</span>
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or
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<span style="color:#4460EF">absence</span>
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of 25 &mu;M SSA. The first set of bars represent the results with </html>[[Part:BBa_K4361000]], second set of bars correspond to those of </html>[[Part:BBa_K4361001]]<html>. The third set depicts the measured fraction of bound DNA for this part.</figcaption>
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</figure>
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</html>
  
  

Revision as of 14:13, 8 October 2022


BlcR-binding oligo, 51 bp, IR1 outer 5 + IR2

Based off of BBa_K4361001. Since that part's second inverted repeat pair consists of only 5 nucleotides on each end of the region, the region marked "Inverted repeat pair 1" was adjusted to only have the outer 5 nucleotides be the inverted repeat while the middlemost nucleotides have been replaced by pair 2's non-repeating sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and biology

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Results

~~~
Figure 2. Results of the second Tapestation experiment, in which the fraction of DNA bound to BlcR was determined for different types of oligos. The first bar and bottom dashed line represent the results with Part:BBa_K4361000 (scrambled oligo, negative control), the second bar and top dashed line correspond to those with Part:BBa_K4361001 (wildtype oligo, positive control). The third bar depicts the measured fraction of bound DNA for this part.
Figure 3. Results of the third Tapestation experiment, in which the fraction of DNA bound to BlcR was determined for different types of oligos in the presence or absence of 25 μM SSA. The first set of bars represent the results with Part:BBa_K4361000, second set of bars correspond to those of </html>Part:BBa_K4361001. The third set depicts the measured fraction of bound DNA for this part.