Difference between revisions of "Part:BBa K4345018"
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The toxin ccdB (including the stop codon control, see BBa_K4345018) is under the control of a promoter that is activated by both anhydrotetracyclin and lacI. ccdB is fused to sfGFP with a rigid linker. | The toxin ccdB (including the stop codon control, see BBa_K4345018) is under the control of a promoter that is activated by both anhydrotetracyclin and lacI. ccdB is fused to sfGFP with a rigid linker. | ||
Latest revision as of 14:09, 8 October 2022
Tet/lac promoter - ccdB fused to sfGFP
The toxin ccdB (including the stop codon control, see BBa_K4345018) is under the control of a promoter that is activated by both anhydrotetracyclin and lacI. ccdB is fused to sfGFP with a rigid linker.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1265
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 352
Illegal AgeI site found at 685 - 1000COMPATIBLE WITH RFC[1000]