Difference between revisions of "Part:BBa K228009"

Revision as of 09:55, 22 September 2009

AraC protein(reversed sequence) and Pbad promoter

This part contains AraC protein generator(BBa_K228008) and Pbad promoter(BBa_I13453). The expression of any coding sequence following the Pbad promoter is repressed constitutively by AraC protein and induced by L-arabinose.

This promoter is placed upstream of a GFP gene to character its induction curve. We cultured the bacteria until the value of OD600 reached 0.4 and then added different concentration of arabinose. The process of inducement lasted two hours. We used flowcytometry to test the fluorescence in order to acquire the induction curve, which is shown in the below graph:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1274
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1214
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This part contains AraC protein generator(<partinfo>BBa_K228008</partinfo>) and Pbad promoter(<partinfo>BBa_I13453</partinfo>). The expression of any coding sequence following the Pbad promoter is repressed constitutively by AraC protein and induced by L-arabinose.
-This promoter is then placed upstream of a GFP gene to get it induction data. We use flowcytometry to test the fluorescence at different concentrations of the inducer arabinose.
+This promoter is placed upstream of a GFP gene to character its induction curve. We cultured the bacteria until the value of OD600 reached 0.4 and then added different concentration of arabinose. The process of inducement lasted two hours. We used flowcytometry to test the fluorescence in order to acquire the induction curve, which is shown in the below graph:
-The Induction curve is shown below:
 [[Image:PKU_AraC.jpg|600px]]