Difference between revisions of "Part:BBa K4345017"
 
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Biosensor circuit in pSynSense2.5000 developed by De Paepe et al. (2018) is composed of TT3-rrnD1-T1, fdeR, rrnB T1 terminator, PFdeAR, and pSynSens2.5000 RBS. The modified LysR-type PfdeAR-FdeR pair from Herbaspirillum seropedicae allows this circuit to respond to naringenin. There are two modules in the circuit: detector and effector. In the detector module, FdeR will produce the transcription factor of the effector module. This transcription factor will bind to the binding site upon the presence of naringenin, thus making this circuit naringenin responsive.
 
Biosensor circuit in pSynSense2.5000 developed by De Paepe et al. (2018) is composed of TT3-rrnD1-T1, fdeR, rrnB T1 terminator, PFdeAR, and pSynSens2.5000 RBS. The modified LysR-type PfdeAR-FdeR pair from Herbaspirillum seropedicae allows this circuit to respond to naringenin. There are two modules in the circuit: detector and effector. In the detector module, FdeR will produce the transcription factor of the effector module. This transcription factor will bind to the binding site upon the presence of naringenin, thus making this circuit naringenin responsive.
  
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4345017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4345017 SequenceAndFeatures</partinfo>
  
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===Usage and Biology===
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This part is part of the biosensor circuit shown below.
  
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===Functional Parameters===
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[[Image:PSynSense2X De Paepe et al 2018.png|500px]]
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<partinfo>BBa_K4345017 parameters</partinfo>
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Image obtained from De Paepe et. al. (2018)
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===References===
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De Paepe, B., Maertens, J., Vanholme, B., & De Mey, M. (2018, April 24). Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor. ACS Synthetic Biology, 7(5), 1303–1314. https://doi.org/10.1021/acssynbio.7b00419

Latest revision as of 13:54, 8 October 2022


pSynSense2.5000 PfdeAR-FdeR biosensor circuit

Biosensor circuit in pSynSense2.5000 developed by De Paepe et al. (2018) is composed of TT3-rrnD1-T1, fdeR, rrnB T1 terminator, PFdeAR, and pSynSens2.5000 RBS. The modified LysR-type PfdeAR-FdeR pair from Herbaspirillum seropedicae allows this circuit to respond to naringenin. There are two modules in the circuit: detector and effector. In the detector module, FdeR will produce the transcription factor of the effector module. This transcription factor will bind to the binding site upon the presence of naringenin, thus making this circuit naringenin responsive.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1058
    Illegal XbaI site found at 1088
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1058
    Illegal NheI site found at 1523
    Illegal NotI site found at 1094
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1058
    Illegal BamHI site found at 1073
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1058
    Illegal XbaI site found at 1088
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 580
    Illegal EcoRI site found at 1058
    Illegal XbaI site found at 1088
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 779
    Illegal NgoMIV site found at 870
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1544

Usage and Biology

This part is part of the biosensor circuit shown below.


PSynSense2X De Paepe et al 2018.png

Image obtained from De Paepe et. al. (2018)

References

De Paepe, B., Maertens, J., Vanholme, B., & De Mey, M. (2018, April 24). Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor. ACS Synthetic Biology, 7(5), 1303–1314. https://doi.org/10.1021/acssynbio.7b00419