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==Contribution: iGEM22_WHU-China ==
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Mistic possesses two features whose relationship has remained unclear: its interaction with the lipid bilayer and its ability to promote the overexpression of heterologous membrane proteins. The results of the article reveal that these two features are not correlated, as they could express the Y4 receptor, a human GPCR, also when fused to only H3 from Mistic.<br>
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                <p style="color:Gray; padding:0px 30px 10px;"> Fig.1 (A) Comparison of maps of vectors pDXW-1, pDXW-2, pDXW-3 and pDXW-3-LRaceE. (B)Construction of the C. glutamicum aceE mutant strain YTW-1. (C and D) Growth difference ofC. glutamicum ATCC13032 and YTW-1 on CGXII media in the presence (C) or absence (D) of sodium acetate. </p>
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Revision as of 13:50, 8 October 2022


Mistic (Mstx)

Mistic (Mstx) is an unique hydrophilic protein from Bacillus subtilis that works as a fusion tag and helps to integrate other proteins into lipid membranes more easily. When fused to the N-terminus of integral membrane proteins, MstX enables the cargo proteins to fold into their native conformations in the membrane, thus yielding a high-level expression. Therefore, this biobrick has RFC12 prefix and suffix that allows to fuse proteins to the N or C-terminus in a stop codon free way.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Contribution: iGEM22_WHU-China

Mistic possesses two features whose relationship has remained unclear: its interaction with the lipid bilayer and its ability to promote the overexpression of heterologous membrane proteins. The results of the article reveal that these two features are not correlated, as they could express the Y4 receptor, a human GPCR, also when fused to only H3 from Mistic.

Fig.1 (A) Comparison of maps of vectors pDXW-1, pDXW-2, pDXW-3 and pDXW-3-LRaceE. (B)Construction of the C. glutamicum aceE mutant strain YTW-1. (C and D) Growth difference ofC. glutamicum ATCC13032 and YTW-1 on CGXII media in the presence (C) or absence (D) of sodium acetate.