Difference between revisions of "Part:BBa K4345012"
 
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Coding sequence of fdeR from PfdeAR-FdeR biosensor circuit's detector module. FdeR is a part of Naringenin-Responsive transcriptional biosensor develop by De Paepe et al. (2018). Native naringenin-responsive regulatory circuit, LysR-type PfdeAR-FdeR pair from ''Herbaspirillum seropedicae'' was redesigned to study the naringenin response in E. coli.  
 
Coding sequence of fdeR from PfdeAR-FdeR biosensor circuit's detector module. FdeR is a part of Naringenin-Responsive transcriptional biosensor develop by De Paepe et al. (2018). Native naringenin-responsive regulatory circuit, LysR-type PfdeAR-FdeR pair from ''Herbaspirillum seropedicae'' was redesigned to study the naringenin response in E. coli.  
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===Usage and Biology===
 
  
 
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===Sequence and Features===
 
===Sequence and Features===
 
<partinfo>BBa_K4345012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4345012 SequenceAndFeatures</partinfo>
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===Usage and Biology===
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This part is part of the biosensor circuit shown below.
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[[Image:PSynSense2X De Paepe et al 2018.png|500px]]
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Image obtained from De Paepe et. al. (2018)
  
 
===References===
 
===References===
  
 
De Paepe, B., Maertens, J., Vanholme, B., & De Mey, M. (2018, April 24). Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor. ACS Synthetic Biology, 7(5), 1303–1314. https://doi.org/10.1021/acssynbio.7b00419
 
De Paepe, B., Maertens, J., Vanholme, B., & De Mey, M. (2018, April 24). Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor. ACS Synthetic Biology, 7(5), 1303–1314. https://doi.org/10.1021/acssynbio.7b00419

Latest revision as of 13:25, 8 October 2022


FdeR

Coding sequence of fdeR from PfdeAR-FdeR biosensor circuit's detector module. FdeR is a part of Naringenin-Responsive transcriptional biosensor develop by De Paepe et al. (2018). Native naringenin-responsive regulatory circuit, LysR-type PfdeAR-FdeR pair from Herbaspirillum seropedicae was redesigned to study the naringenin response in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 466
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 466
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 466
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 466
    Illegal NgoMIV site found at 653
    Illegal NgoMIV site found at 665
    Illegal NgoMIV site found at 756
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

This part is part of the biosensor circuit shown below.


PSynSense2X De Paepe et al 2018.png

Image obtained from De Paepe et. al. (2018)

References

De Paepe, B., Maertens, J., Vanholme, B., & De Mey, M. (2018, April 24). Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor. ACS Synthetic Biology, 7(5), 1303–1314. https://doi.org/10.1021/acssynbio.7b00419