Difference between revisions of "Part:BBa K4239001:Design"

Revision as of 13:03, 8 October 2022

Enhanced luciferase subunits fiatluxC

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1311

Design Notes

The Igem restriction site Pst1 was removed into the iluxC part, to create fiatluxC. This mutation does not change the protein sequence.

iluxC had mutations from luxC (N10T, N59D, E74D, S256P, M355T, N360D). hey change the protein sequence, in order to create a system that produced more light. The first and the last letters correspond to the Amino Acid before and after mutation, and the number indicats its position. These mutations were errore-prone PCR induced according to Gregor et al.’s study in 2018.

Source

fiatluxCD is made up of fiatluxC and fiatluxD.

References

Gregor C, Gwosch KC, Sahl SJ, Hell SW. Strongly enhanced bacterial bioluminescence with the ilux operon for single-cell imaging. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):962-967. doi: 10.1073/pnas.1715946115. Epub 2018 Jan 16. PMID: 29339494; PMCID: PMC5798359.

@@ Line 9: / Line 9: @@
 The Igem restriction site Pst1 was removed into the iluxC part, to create fiatluxC. This mutation does not change the protein sequence.
-iluxC had mutations from luxC (N10T, N59D, E74D, S256P, M355T, N360D). These mutations were errore-prone PCR induced according to Gregor et al.’s study in 2018. They change the protein sequence, in order to create a system that produced more light.
+iluxC had mutations from luxC (N10T, N59D, E74D, S256P, M355T, N360D). hey change the protein sequence, in order to create a system that produced more light. The first and the last letters correspond to the Amino Acid before and after mutation, and the number indicats its position. These mutations were errore-prone PCR induced according to Gregor et al.’s study in 2018.
 ===Source===