Difference between revisions of "Part:BBa K4345010:Design"
| Line 11: | Line 11: | ||
===Source=== | ===Source=== | ||
| − | L. monocytogenes virulence modulation, often via cross-talk with PrfA, or by regulating new genes involved in host colonization. | + | ''L. monocytogenes'' virulence modulation, often via cross-talk with PrfA, or by regulating new genes involved in host colonization. |
===References=== | ===References=== | ||
Johansson J, Mandin P, Renzoni A, Chiaruttini C, Springer M, Cossart P. An RNA thermosensor controls expression of virulence genes in Listeria monocytogenes. Cell. 2002;110(5):551-561. doi:10.1016/s0092-8674(02)00905-4 | Johansson J, Mandin P, Renzoni A, Chiaruttini C, Springer M, Cossart P. An RNA thermosensor controls expression of virulence genes in Listeria monocytogenes. Cell. 2002;110(5):551-561. doi:10.1016/s0092-8674(02)00905-4 | ||
Latest revision as of 12:58, 8 October 2022
RNA thermometer prfA
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Our team used the RNA thermometer as a part of a killswitch. The sequence was implemented before an antitoxin (ccdB) to regulate its translation based on temperature. It is possible to implement a part of the sequence of the gene that will be translated within the sequence that forms secondary hairpin structure. By doing this, leaky expression will be reduced. Because of the typical short sequence of this thermometer and the position of the startcodon, our team decided not to. The position of the startcodon can be found in the provided figure.
Source
L. monocytogenes virulence modulation, often via cross-talk with PrfA, or by regulating new genes involved in host colonization.
References
Johansson J, Mandin P, Renzoni A, Chiaruttini C, Springer M, Cossart P. An RNA thermosensor controls expression of virulence genes in Listeria monocytogenes. Cell. 2002;110(5):551-561. doi:10.1016/s0092-8674(02)00905-4