Difference between revisions of "Part:BBa K4345010"
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RNA thermometer used for post-transcriptional regulation based on a temperature-dependent system, based on a RNA thermometer from Listeria monocytogenes. It is maximally expressed at 37ºC and almost not detectable at 30ºC. It operates as a reversible molecular zipper that controls the availability of the RBS in its structure. When the temperature increases, the thermometer unzips and the RBS can be detected by the ribosome thus allowing translation of downstream mRNA. When the temperature is below a certain range, the RBS gets trapped.
 
RNA thermometer used for post-transcriptional regulation based on a temperature-dependent system, based on a RNA thermometer from Listeria monocytogenes. It is maximally expressed at 37ºC and almost not detectable at 30ºC. It operates as a reversible molecular zipper that controls the availability of the RBS in its structure. When the temperature increases, the thermometer unzips and the RBS can be detected by the ribosome thus allowing translation of downstream mRNA. When the temperature is below a certain range, the RBS gets trapped.
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===Sequence and Features===
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<partinfo>BBa_K4345010 SequenceAndFeatures</partinfo>
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===Usage and Biology===
 
===Usage and Biology===
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The figure below shows the secondary structure of the RNA thermometer.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4345010 SequenceAndFeatures</partinfo>
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[[Image:PrfA Johansson et al 2002.jpeg|300px]]
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===Functional Parameters===
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Image obtained from Johansson et al., 2002
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<partinfo>BBa_K4345010 parameters</partinfo>
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===References===
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Johansson, J., Mandin, P., Renzoni, A., Chiaruttini, C., Springer, M., & Cossart, P. (2002, September). An RNA Thermosensor Controls Expression of Virulence Genes in Listeria monocytogenes. Cell, 110(5), 551–561. https://doi.org/10.1016/s0092-8674(02)00905-4

Revision as of 12:55, 8 October 2022


RNA thermometer prfA

RNA thermometer used for post-transcriptional regulation based on a temperature-dependent system, based on a RNA thermometer from Listeria monocytogenes. It is maximally expressed at 37ºC and almost not detectable at 30ºC. It operates as a reversible molecular zipper that controls the availability of the RBS in its structure. When the temperature increases, the thermometer unzips and the RBS can be detected by the ribosome thus allowing translation of downstream mRNA. When the temperature is below a certain range, the RBS gets trapped.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]