Difference between revisions of "Part:BBa K4204016"

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This part contains a coding sequence of sfGFP (BBa_K515105) with N-terminal His tag from pET28a(+). It is intended to be used to characterize the crystal violet induction system in our project. The N-terminal His tag mimics the realistic protein expression condition of pET28a(+).
 
  
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<!-- Uncomment this to enable Functional Parameter display  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K4204016 parameters</partinfo>
 
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Revision as of 12:36, 8 October 2022

Crystal violet induced sfGFP



Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 907
    Illegal PstI site found at 456
    Illegal PstI site found at 792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 907
    Illegal NheI site found at 1005
    Illegal PstI site found at 456
    Illegal PstI site found at 792
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 907
    Illegal BglII site found at 916
    Illegal XhoI site found at 1728
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 907
    Illegal PstI site found at 456
    Illegal PstI site found at 792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 907
    Illegal PstI site found at 456
    Illegal PstI site found at 792
    Illegal NgoMIV site found at 477
    Illegal AgeI site found at 327
    Illegal AgeI site found at 1158
  • 1000
    COMPATIBLE WITH RFC[1000]