Difference between revisions of "Part:BBa K4164002"

Revision as of 12:27, 8 October 2022

farnesoid X receptor(FXR)

FXR is a protein from humans, it is a member of the nuclear receptor superfamily of ligand-activated transcription factors， and functions as a receptor for bile acids. It is encoded by the NR1H4 in human. FXR is expressed at high levels in the liver and intestine.

Chenodeoxycholic acid （CDCA） and other bile acids are natural ligands for FXR. Similar to other nuclear receptors, when activated, FXR translocates to the cell nucleus, forms a dimer (in this case a heterodimer with RXR) and binds to hormone response elements on DNA, which up- or down-regulates the expression of certain genes.

In our project, we took advantage of the characteristics of CDCA as the most effective activator of FXR, using FXR as the receptor, and when activated by de-sulfated CDCA-S, it will form a heterodimeric complex with the RXR, so that the linked ddRFP-A1 and ddRFP-B1 are correspondingly driven to dimerize and emit fluorescence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1057

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4164002 short</partinfo>
-FXR is a protein from humans, it is a member of the nuclear receptor superfamily of ligand-activated transcription factors， and functions as a receptor for bile acids. It is encoded by the NR1H4 gene in humans. FXR is expressed at high levels in the liver and intestine.
+FXR is a protein from humans, it is a member of the nuclear receptor superfamily of ligand-activated transcription factors， and functions as a receptor for bile acids. It is encoded by the <em>NR1H4</em> in human. FXR is expressed at high levels in the liver and intestine.
 Chenodeoxycholic acid （CDCA） and other bile acids are natural ligands for FXR. Similar to other nuclear receptors, when activated, FXR translocates to the cell nucleus, forms a dimer (in this case a heterodimer with RXR) and binds to hormone response elements on DNA, which up- or down-regulates the expression of certain genes.
-In our project, we took advantage of the characteristics of CDCA as the most effective activator of FXR, using FXR as the receptor, and when activated by de-sulfated CDCA, it will form a heterodimeric complex with the RXR, so that the downstream ddRFP-A1 and ddRFP-B1 are correspondingly driven to dimerize and emit fluorescence.
+In our project, we took advantage of the characteristics of CDCA as the most effective activator of FXR, using FXR as the receptor, and when activated by de-sulfated CDCA-S, it will form a heterodimeric complex with the RXR, so that the linked ddRFP-A1 and ddRFP-B1 are correspondingly driven to dimerize and emit fluorescence.
 <!-- Add more about the biology of this part here