Difference between revisions of "Part:BBa K4244010"

 
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__NOTOC__
 
__NOTOC__
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{|width='80%'
 
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|-
 
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|width=35% valign='top'|
 
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[[Image:Terminator.png]]
 
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<partinfo>BBa_K4244010 parameters</partinfo>
 
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| width=50% valign='top' style='border: 1px solid black'|
 
 
<partinfo>BBa_K4244010 short</partinfo>
 
<partinfo>BBa_K4244010 short</partinfo>
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* Terminator from phage Lambda
 
  
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|}
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The Bba_0100 terminator from the registry was combined with a specific 3'UTR region of 30 nucleotides, that cause for a high protein expression.
  
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'''Secondary Structure'''
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4244010 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K4244010 parameters</partinfo>
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<!-- -->
  
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[[Image:Mfold-K4244010-1.png]]
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===References===
  
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<hr>
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Chapter 5 of thesis Thijs Nieuwkoop, Translational feed-forward andfeed-back control, Sjoerd C.A. Creutzburg*,Thijs Nieuwkoop*, Thijmen Zegers & John van der Oost.
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'''Measurement'''
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* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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Revision as of 12:11, 8 October 2022


Terminator with 3' spacer region (#9) for high protein production

The Bba_0100 terminator from the registry was combined with a specific 3'UTR region of 30 nucleotides, that cause for a high protein expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Chapter 5 of thesis Thijs Nieuwkoop, Translational feed-forward andfeed-back control, Sjoerd C.A. Creutzburg*,Thijs Nieuwkoop*, Thijmen Zegers & John van der Oost.