Difference between revisions of "Part:BBa K4193027:Design"

 
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===References===
 
===References===
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Yang, X., Liu, J., Zhang, J., Shen, Y., Qi, Q., Bao, X. and Hou, J. (2021). Quorum sensing-mediated protein degradation for dynamic metabolic pathway control in Saccharomyces cerevisiae. Metabolic Engineering, 64, pp.85–94. doi:10.1016/j.ymben.2021.01.010.

Revision as of 11:00, 8 October 2022


quorum sensing system (two parts)


Assembly Compatibility:
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    Illegal EcoRI site found at 4719
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    COMPATIBLE WITH RFC[1000]


Design Notes

we use cytokinin-mediated quorum sensing circuits from Arabidopsis thaliana makes our engineered microorganisms only reaches a certain population density will start fermentation process, used for decoupling competition between cell growth and fermentation. Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. This Quorum-sensing systems were used to test the effect of IP concentration on the expression of target genes, and thus AtlPT4 was not available. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter and initiates transcription.


Source

synthesize from company

References

Yang, X., Liu, J., Zhang, J., Shen, Y., Qi, Q., Bao, X. and Hou, J. (2021). Quorum sensing-mediated protein degradation for dynamic metabolic pathway control in Saccharomyces cerevisiae. Metabolic Engineering, 64, pp.85–94. doi:10.1016/j.ymben.2021.01.010.