Difference between revisions of "Part:BBa K4197013"
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<h2>Construction</h2> | <h2>Construction</h2> | ||
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Revision as of 10:57, 8 October 2022
mTagBFP under control of ihfB800 promoter
Brick expressing BFP fluorescent protein with ihfB800 promoter.
Introduction
This part is composed of the gene coding for the 800 first bp of the ihfB promoter. This promoter has been identified as a constitutive E. coli promoter (Weglenska et al., 1996). It is often used by researchers of the Toulouse Biotechnology Institute to express recombinant fluorescent proteins in E. coli (Barthe et al., 2020), as it is strong enough to allow correct expression and weak enough to avoid inclusion bodies. BFP protein was expressed using this promoter
Construction
Xxxxx xxxxx xxxxx xxxxxx xxxx