Difference between revisions of "Part:BBa K4140027"
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Revision as of 10:09, 8 October 2022
Permeability device
Part Description
In this composite Lac promoter regulate the expression of phenylalanine permease that is a single, integral membrane transporter that uses the proton motive force to antiport L-phenylalanine and L-tyrosine. And present naturally in Escherichia coli.
Usage
We implement this composite to increase the permeability of cells to phenylalanine by the expression of phenylalanine permease that is a single, integral membrane transporter to improve the sensitivity of our circuit to phenylalanine as shown in figure 1 and figure 2.
Figure 1. (shows the permeability circuit in our E.coli-based system.)
Figure 2. (shows the whole cell-based biosensor.)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 81
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]