Difference between revisions of "Part:BBa K4413993"
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gabY is the gene used for promotes the combination of PQQ coenzyme and GDH, increases the effective concentration of the holoenzymes, enhances GDH’s catalytic efficiency, and we insert RiboJ between promoter and RBS to increase Translation efficiency.With the over-expression of gabY, it can decrease the pH to a greater extent and promote the dissolution of phosphate. | gabY is the gene used for promotes the combination of PQQ coenzyme and GDH, increases the effective concentration of the holoenzymes, enhances GDH’s catalytic efficiency, and we insert RiboJ between promoter and RBS to increase Translation efficiency.With the over-expression of gabY, it can decrease the pH to a greater extent and promote the dissolution of phosphate. | ||
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| + | <h2>Introduction</h2> | ||
| + | BBa_K3560005 contains RBS and gabY genes. We plan to improve this part, and constructed the improved part (BBa_K4413993). We inserted a RiboJ sequence in the upstream of gabY and downstream of the PGroES promoter. In addition, we described the functions of this part in Deinococcus radiodurans (DR), including the growth curve and the ability to decrease pH and dissolve phosphate. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 10:03, 8 October 2022
PGroES-RiboJ-DrRBS-gabY
gabY is the gene used for promotes the combination of PQQ coenzyme and GDH, increases the effective concentration of the holoenzymes, enhances GDH’s catalytic efficiency, and we insert RiboJ between promoter and RBS to increase Translation efficiency.With the over-expression of gabY, it can decrease the pH to a greater extent and promote the dissolution of phosphate.
Introduction
BBa_K3560005 contains RBS and gabY genes. We plan to improve this part, and constructed the improved part (BBa_K4413993). We inserted a RiboJ sequence in the upstream of gabY and downstream of the PGroES promoter. In addition, we described the functions of this part in Deinococcus radiodurans (DR), including the growth curve and the ability to decrease pH and dissolve phosphate.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 476
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 190
Illegal NgoMIV site found at 390
Illegal NgoMIV site found at 482
Illegal NgoMIV site found at 648 - 1000COMPATIBLE WITH RFC[1000]