Difference between revisions of "Part:BBa K4147005"
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This part contains the CDS of the antimicrobial peptide Dermaseptin B1 (BBa_K2577001) under the promotion of a LacI regulated promoter (BBa_R0010). This construct also contains an RBS under the part name BBa_B0032 as well as a 6xHis tag on the C- terminal for purification with IMAC and lastly a double terminator (BBa_B0015).
 
This part contains the CDS of the antimicrobial peptide Dermaseptin B1 (BBa_K2577001) under the promotion of a LacI regulated promoter (BBa_R0010). This construct also contains an RBS under the part name BBa_B0032 as well as a 6xHis tag on the C- terminal for purification with IMAC and lastly a double terminator (BBa_B0015).
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===Usage and Biology===
  
 
This synthetic derivative of dermaseptin B1, MsrA2 (N-Met-dermaseptin B1) has proven to possess activity against phytopathogenic fungi such as <i>Alternaria</i>, <i>Cercospora </i>, <i>Fusarium</i>, <i>Phytophthora</i>, <i>Pythium</i>, <i>Rhizoctonia</i> and <i>Verticillium</i> sp [1].
 
This synthetic derivative of dermaseptin B1, MsrA2 (N-Met-dermaseptin B1) has proven to possess activity against phytopathogenic fungi such as <i>Alternaria</i>, <i>Cercospora </i>, <i>Fusarium</i>, <i>Phytophthora</i>, <i>Pythium</i>, <i>Rhizoctonia</i> and <i>Verticillium</i> sp [1].

Revision as of 09:36, 8 October 2022

Dermaseptin B1 (AKA MsrA2) expression construct with LacI regulated promoter

This part contains the CDS of the antimicrobial peptide Dermaseptin B1 (BBa_K2577001) under the promotion of a LacI regulated promoter (BBa_R0010). This construct also contains an RBS under the part name BBa_B0032 as well as a 6xHis tag on the C- terminal for purification with IMAC and lastly a double terminator (BBa_B0015).

Usage and Biology

This synthetic derivative of dermaseptin B1, MsrA2 (N-Met-dermaseptin B1) has proven to possess activity against phytopathogenic fungi such as Alternaria, Cercospora , Fusarium, Phytophthora, Pythium, Rhizoctonia and Verticillium sp [1].