Difference between revisions of "Part:BBa K4271004"

Revision as of 09:31, 8 October 2022

pNP Mut 1-1

The pNP Mut 1-1 is a gene that encodes the activation protein for downstream GFP expressions. After adding pNP to the sensor plasmid BL21, it binds to the pNP Mut 1-1 protein and acts as a transcriptional factor for GFP. In our experiment, we measure the level of GFP fluorescence to determine the success of paraoxon degradation. After the pNP Mut 1-1 protein is used, the pNP Mut 1-1 gene is expressed again, continuing the cycle.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 672
1000
COMPATIBLE WITH RFC[1000]

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+<center><figure style=" width: 20%; text-align: center; font-style: italic; font-size: smaller; text-indent: 0 ">
+         <img src="https://static.igem.wiki/teams/4271/wiki/pnpmut1-1.png" width=100% style="border: 1px solid black;">
+    </figure></center>
+</html>
 The pNP Mut 1-1 is a gene that encodes the activation protein for downstream GFP expressions. After adding pNP to the sensor plasmid BL21, it binds to the pNP Mut 1-1 protein and acts as a transcriptional factor for GFP. In our experiment, we measure the level of GFP fluorescence to determine the success of paraoxon degradation. After the pNP Mut 1-1 protein is used, the pNP Mut 1-1 gene is expressed again, continuing the cycle.