Difference between revisions of "Part:BBa K4043004"

 
Line 17: Line 17:
 
<partinfo>BBa_K4043004 parameters</partinfo>
 
<partinfo>BBa_K4043004 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
==User Reviews==
 +
===Contribution from iGEM 2022 ZJU-China===
 +
Group: ZJU-China 2022
 +
<br>
 +
Author: Zhijian Yan
 +
<br>
 +
Summary: In 2022, ZJU-China used this part in their project to design a kill switch regulated by the concentration of sucrose in the media. The kill switch containing this promoter has been proved to work very well in Bacillus subtilis, but our characterization result also indicated that the promoter activity is very low and its activity did not rise 100-fold when the inducer existed.

Revision as of 09:24, 8 October 2022


A weak promoter

The transcription of the PsacB promoter is not strictly regulated by sucrose, and can be transcribed without an inducer, but the intensity is 100 times lower than that of sucrose induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


User Reviews

Contribution from iGEM 2022 ZJU-China

Group: ZJU-China 2022
Author: Zhijian Yan
Summary: In 2022, ZJU-China used this part in their project to design a kill switch regulated by the concentration of sucrose in the media. The kill switch containing this promoter has been proved to work very well in Bacillus subtilis, but our characterization result also indicated that the promoter activity is very low and its activity did not rise 100-fold when the inducer existed.