Difference between revisions of "Part:BBa K4345000:Design"

Revision as of 09:16, 8 October 2022

NarX fused to mNeonGreen with a rigid linker

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 659
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 659
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 260
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 659
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 659
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2236

Design Notes

NarX and mNeonGreen are fused with a rigid linker. This allows for an independent folding of both proteins.

Source

NarX is derived from E. coli genome, mNeonGreen is derived from Branchiostoma lanceolatum.

NarX sequence was obtained from NCBI and the sequence of mNeonGreen from the iGEM Registry of Standard Biological Parts.

References

narX sensor histidine kinase NarX [ Escherichia coli str. K-12 substr. MG1655 ]. (2022, September 22). National Library of Medicine - National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/gene/945788

Cavicchioli, R., Schro¨der, I., Schro¨der, S., Constanti, M., & Gunsalus, R. P. (1995). The NarX and NarQ Sensor-Transmitter Proteins of Escherichia coli Each Require Two Conserved Histidines for Nitrate-Dependent Signal Transduction to NarL. JOURNAL OF BACTERIOLOGY, 177(9), 2416–2424.

Cheung, J., & Hendrickson, W. A. (2009). Structural Analysis of Ligand Stimulation of the Histidine Kinase NarX. Structure, 17(2), 190–201. https://doi.org/10.1016/J.STR.2008.12.013

@@ Line 13: / Line 13: @@
 ===Source===
-NarX is derived from E. coli genome, mNeonGreen is derived from Branchiostoma lanceolatum
+NarX is derived from E. coli genome, mNeonGreen is derived from ''Branchiostoma lanceolatum''.
 NarX sequence was obtained from NCBI and the sequence of mNeonGreen from the iGEM Registry of Standard Biological Parts.